LGMN Protéine

N° du produit ABIN7964651

Cette protéine Recombinant LGMN est produite dans Leishmania tarentolae.

Aperçu rapide pour LGMN Protéine (ABIN7964651)

Antigène: LGMN (Legumain (LGMN))

Type de proteíne: Recombinant

Origine: Humain

Source: Leishmania tarentolae

Pureté: > 90 % (SDS-PAGE)

Détail du produit

Fonction: Legumain, Asparaginyl Endopeptidase (AEP), human, recombinant, Leishmania

Attributs du produit: Legumain is a cysteine protease with a strict specificity for cleaving after asparagine and, to a lesser extent, aspartic acid residues. Therefore, it is synonymously named the asparaginyl endopeptidase or AEP. Its very strict substrate specificity makes it an ideal enzyme for example for digestion proteomics. Human legumain is synthesized as an inactive proenzyme (56 kDa) composed of the caspase-like catalytic AEP domain (36 kDa) and a C-terminal death-domain-like prodomain. Activation to the active AEP proceeds via the pH -dependent autocatalytic release of the C-terminal prodomain at acidic pH . While prolegumain is stable at neutral pH , AEP is stable at acidic pH (≤ 6.0) but will irreversibly unfold at near neutral pH conditions. The pH -optimum of its endopeptidase activity is at pH 5.5 with a strong preference for cleaving after asparagine residues. Hydrolysis of aspartyl peptide bonds is preferentially catalyzed at more acidic pH ( pH 4.0). In addition to its well established protease activity, legumain harbors a pH -dependent ligase activity at near neutral pH .

Information d'application

Commentaires:

Optional[Concentration Comments]: 500 μg/mL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: supplied in 20 mM citric acid pH 4.0100 mM NaCl2 mM DTT

Agent conservateur: Dithiothreitol (DTT)

Précaution d'utilisation: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Please centrifuge briefly before opening (volume ≤2 ml).,avoid freeze/thaw cycles

Stock: -20 °C

Stockage commentaire: store at -20 °C avoid freeze/thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: LGMN (Legumain (LGMN))

Autre désignation: Legumain

Poids moléculaire: 36 kDa

UniProt: Q99538

Pathways: Metabolism of Steroid Hormones and Vitamin D, Activation of Innate immune Response, Toll-Like Receptors Cascades