IFN alpha 2b Protein (AA 24-188)

N° du produit ABIN8056968

Cette protéine Recombinant IFN alpha 2b est produite dans Escherichia coli (E. coli).

Aperçu rapide pour IFN alpha 2b Protein (AA 24-188) (ABIN8056968)

Antigène: IFN alpha 2b

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: Cell Culture (CC)

Pureté: > 95 % as determined by reducing SDS-PAGE.

Détail du produit

Attributs du protein: AA 24-188

Fonction: Recombinant Human IFNα2b/IFNA2 Protein

Séquence: Cys24-Glu188

Stérilité: 0.2 μm filtered

niveau d'endotoxine: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured in antiviral assay using A549 human lung cancer cells infected with vesicular stomatitisvirus (VSV) The ED₅₀ for this effect is 5 ng/mL.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 12 months

Détail du antigène

Antigène: IFN alpha 2b

Autre désignation: IFNalpha2b

Sujet: IFN-Alpha-2,IFN-alpha 2b,IFNA2,Interferon Alpha-2,Interferon Alpha-A,LeIF A,At least 23 different variants of IFN-α are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-α subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-α subtypes differ in their sequences by only one or two positions. Naturally occurring variants also include proteins that are truncated by 10 amino acids at the carboxyl-terminal end.

Poids moléculaire:

Calculated MW: 19.4 kDa

Observed MW: 17 kDa

ID gène: 3440

UniProt: P01563