HIV-1 Tat Protéine

N° du produit ABIN934615

Détail du produit

Antigène: HIV-1 Tat

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Human Immunodeficiency Virus (HIV)

Source: Escherichia coli (E. coli)

Application: ELISA

Attributs du produit: Purified recombinant HIV1 tat protein
Expression System: E.coli
Bioactivity: Tat concentrations in 1 µg/mL range produced at least 25 fold increase in CAT activity. The biological activity of this protein was determined by LTR-CAT activation in scrapie-loaded HeLe Cells, and in U373 MG cells in the presence of 100uM chlorochine diphosphate.

Purification: Ion affinity and reverse phase HPLC

Pureté: > 99 % pure

Information d'application

Indications d'application: Each Investigator should determine their own optimal working dilution for specific applications.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Supplied as a frozen liquid. Dilute tat stock solution in 150 mM NaCl, 50 mM Sodium Citrate, pH 6.5 immediately before use. Tat readily oxidizes in buffer solutions, which may change its LTR dependent transcriptional activation activity.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: -80 °C

Stockage commentaire: Aliquot and store at -70 °C or lower.

Référence

Argyris, Kulkosky, Meyer, Xu, Mukhtar, Pomerantz, Williams: "The perlecan heparan sulfate proteoglycan mediates cellular uptake of HIV-1 Tat through a pathway responsible for biological activity." dans: Virology, Vol. 330, Issue 2, pp. 481-6, (2004) (PubMed).

Détail du antigène

Antigène: HIV-1 Tat

Autre désignation: HIV1 tat (HIV-1 Tat Produits)

Classe de substances: Viral Protein

Sujet: Human immunodeficiency virus (HIV) is a lentivirus that causes acquired immunodeficiency syndrome (AIDS), a condition in humans in which the immune system begins to fail, leading to life-threatening opportunistic infections. HIV has several major genes coding for structural proteins that are found in all retroviruses, and several nonstructural (accessory) genes that are unique to HIV.
Alternative Names: HTATIP1 protein, HIV1 tat, Transactivating regulatory protein protein , HIV 1 tat protein, PLIP protein, HIV-1 tat protein, Human tat interactive protein mRNA complete cds protein, HIV-1 tat protein, HIV 1 Tat interacting protein protein, 2interacting protein protein 60 kDa Tat interactive protein protein cPLA protein cPLA2 protein EC 2.3.1.48 protein ESA1 protein Histone acetyltransferase HTATIP protein HIV 1 Tat interacting protein 60kDa protein, HIV 1 tat, TIP60 protein, Tat interactive protein (60kD) protein, protein Tat protein, TIP protein, HIV-1 tat, HIV 1 tat protein