Glucose-6-Phosphate Dehydrogenase Protein (G6PD) (AA 1-491)

N° du produit ABIN935018

Cette protéine Recombinant Glucose-6-Phosphate Dehydrogenase est produite dans Escherichia coli (E. coli).

Aperçu rapide pour Glucose-6-Phosphate Dehydrogenase Protein (G6PD) (AA 1-491) (ABIN935018)

Antigène: Glucose-6-Phosphate Dehydrogenase (G6PD)

Type de proteíne: Recombinant

Activité biologique: Active

Origine: E. coli

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: > 90 % pure

Détail du produit

Attributs du protein: AA 1-491

Séquence: MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVD SITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E

Attributs du produit: Purified recombinant E.coli G6 PD protein
Expression System: E.coli
Bioactivity: Specific activity is 8-10 units/mL obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP. One unit oxidizes 1.0 m D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25 °C.
Molecular weight on SDS-PAGE will appear higher.

Information d'application

Indications d'application: G6PD protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Procédure de l'essai:

1. Prepare a 3 mL reaction cocktail into a suitable container: The final concentrations are 53 mM gylcylglycine, 3 mM D-glucose 6-phosphate, 0.63 mM beta-NADP, 13 mM MgCl2.
   2. Equilibrate to 25 °C and monitor at A340 nm until the value is constant using a spectrophotometer.
   3. Add 4 µg of recombinant G6PD into reaction cocktail and mix immediately.
   4. Record the increase at A340 nm for 5 minutes.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 50 mM MES 6.0, 0.0 mM PMSF, 0 mM EDTA, 0.0 mM DTT, and 10 % glycerol.

Agent conservateur: Dithiothreitol (DTT)

Précaution d'utilisation: This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: RT/-20 °C

Stockage commentaire: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Détail du antigène

Antigène: Glucose-6-Phosphate Dehydrogenase (G6PD)

Autre désignation: G6PD

Sujet: Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. G6PD converts glucose-6-phosphate into 6-phosphogluconolactone and simultaneously produces NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency cause acute hemolytic anemia. Recombinant G6PD protein was expressed in E. coli and purified by conventional chromatography techniques.
Alternative Names: Glucose 6 phosphate 1 dehydrogenase protein, G6PD1 protein, zwf protein, GPD 6, Glucose-6-phosphate 1-dehydrogenase G6PD protein, Glucose-6-phosphate 1-dehydrogenase protein, G6PD, POS10 protein, GPD-6, GPD-6 protein, MET19 protein, GPD 6 protein, G6pdx protein, Zwf1p.

Poids moléculaire: 55.7 kDa (491 AA)

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones