GSTA1 Protein (AA 1-222)

N° du produit ABIN935078

Cette protéine Recombinant GSTA1 est produite dans Escherichia coli (E. coli).

Aperçu rapide pour GSTA1 Protein (AA 1-222) (ABIN935078)

Antigène: GSTA1 (Glutathione S-Transferase alpha 1 (GSTA1))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: > 90 % pure

Détail du produit

Attributs du protein: AA 1-222

Séquence: MAEKPKLHYF NARGRMESTR WLLAAAGVEF EEKFIKSAED LDKLRNDGYL MFQQVPMVEI DGMKLVQTRA ILNYIASKYN LYGKDIKERA LIDMYIEGIA DLGEMILLLP VCPPEEKDAK LALIKEKIKN RYFPAFEKVL KSHGQDYLVG NKLSRADIHL VELLYYVEEL DSSLISSFPL LKALKTRISN LPTVKKFLQP GSPRKPPMDE KSLEEARKIF RF

Attributs du produit: Purified recombinant Human GSTA1 protein
Expression System: E.coli
Bioactivity: Specific activity is 5-8 units/mg, and is defined as the amount of enzyme that conjugate 1.0 uM of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25 °C.

Information d'application

Indications d'application: GSTA1 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Procédure de l'essai:

1. Prepare a 1 mL reaction mix into a suitable container: The final concentrations are 97 mM potassium phosphate, 0.97 mM EDTA,
   2.5 mM glutathione, reduced, 1.0 mM 1-chloro-2,4-dinitrobenzene (CDNB),
   3.2 % (v/v) ethanol.
   2. Equilibrate to 25 °C and monitor at A340 nm until the value is constant using a spectrophotometer.
   3. Add 50 µL of recombinant GSTA1 protein with various concentrations (1 µg, 1 µg, 1 µg) in 950 µL reaction buffer.
   4. Mix by inversion and record the increase at A340 nm for 5 minutes.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 0 mM DTT and 10 % glycerol.

Agent conservateur: Dithiothreitol (DTT)

Précaution d'utilisation: This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles.

Stock: RT/-20 °C

Stockage commentaire: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Détail du antigène

Antigène: GSTA1 (Glutathione S-Transferase alpha 1 (GSTA1))

Autre désignation: GSTA1

Sujet: GSTA1, also known as Glutathione S-transferase alpha 1, belongs to a glutathione S-transferase alpha class and is the most abundantly expressed in liver. This protein functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. Recombinant human GSTA1 protein was expressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names: GSTA-1 protein, GSTA 1 protein, HA subunit 1., GTH1 protein, Glutathione S-transferase alpha 1 Glutathione S transferase A1 protein, GSTA1 1 protein, GSTA1, GSTA-1, GST epsilon protein, GSTA1-1 protein, GST2 protein, GSTA 1, GSTA1 protein

Poids moléculaire: 25.6 kDa (222 AA)