Purified recombinant Human SCGF beta protein Expression System: E.coli Bioactivity: Determined by its inhibitory effect on the proliferation of TF-1 cells previously grown in media containing GM-CSF. The ED50 for this effect was observed at 7 ng/mL.
Pureté
> 98 % pure
Stérilité
Sterile filtered
niveau d'endotoxine
< 0.1 ng per µg (1 EU/µg).
Indications d'application
Each Investigator should determine their own optimal working dilution for specific applications.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute with water to a concentration of 5 g/mL.
Buffer
Lyophilized from sterile filtered 0.25X PBS with 1 mM DTT and no additives.
Agent conservateur
Dithiothreitol (DTT)
Précaution d'utilisation
This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freeze/thaw cycles.
Stock
4 °C/-20 °C
Stockage commentaire
Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
Antigène
SCGF beta
Sujet
SCGF a and beta are hematopoietic growth factor that exerts their activity at early stages of hematopoiesis. The SCGFs are non-glycosylated species-specific cytokines that can support growth of primitive hematopoietic cells, and in combination with EPO or GM-CSF, promote proliferation or erthroid or myeloid progenitors, respectively. Alternative Names: Stem Cell Growth Factor-beta protein