Protein G Protein (Alkaline Phosphatase (AP))
Aperçu rapide pour Protein G Protein (Alkaline Phosphatase (AP)) (ABIN964531)
Antigène
Voir toutes Protein G ProtéinesType de proteíne
Origine
Hôte
Application
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Purification/Conjugué
- Cette Protein G protéine est marqué à la Alkaline Phosphatase (AP).
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N° du produit (Fournisseur)
- pg00-05
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Fournisseur
- Rockland
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Specificité
- Protein G Alkaline Phosphatase Conjugated was prepared from chromatographically pure Protein G. Protein G Alkaline Phosphatase Conjugated assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Protein G. No reaction was observed against anti-Protein A.
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Indications d'application
- Protein G Alkaline Phosphatase Conjugated is suitable as a detection agent for primary antibodies that are of the IgG isotype. With the addition of pNPP in ELISA experiments, a yellow product is generated that can be detected on a plate reader.
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Commentaires
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Protein G Alkaline Phosphatase Conjugated is suitable as a detection agent for primary antibodies that are of the IgG isotype. With the addition of pNPP in ELISA experiments, a yellow product is generated that can be detected on a plate reader.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1.0 mg/mL
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Buffer
- 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C
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Date de péremption
- 12 months
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- Protein G
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Sujet
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Protein G is a surface protein of two groups of Streptococcal bacteria that has the ability to bind immunoglobulins. Similar to Protein A, but with slightly different specificity, Protein G is an important agent in the purification of proteins due to its ability to bind the Fc region. Protein G also binds to albumin. Alkaline Phosphatase is an enzyme which removes phosphate groups from a variety of substate molecules. As the name implies, this enzyme functions best under basic pH. Alkaline Phosphatase can be utilized in molecular biology in DNA ligation experiments (keeping the DNA linear), radiolabeling preparations, and a detection mediator in ELISA experiments. Protein G Alkalline Phosphatase Conjugated is ideal for investigators involved in Signal Transduction, Molecular Biology, Cancer, and Cell Signaling.
Synonyms: ProG, Streptococcus G protein, ALP, Alk Phos
Antigène
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