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Protein G Protein (Alkaline Phosphatase (AP))

Protéine Recombinant Protein G exprimée dans .
Rockland
N° du produit ABIN964531
N° du produit (Fournisseur): pg00-05

Aperçu rapide pour Protein G Protein (Alkaline Phosphatase (AP)) (ABIN964531)

Antigène

Voir toutes Protein G Protéines
Protein G

Type de proteíne

Recombinant

Origine

Streptococcus

Hôte

Veuillez nous consulter SVP

Application

ELISA
  • Purification/Conjugué

    Cette Protein G protéine est marqué à la Alkaline Phosphatase (AP).

    N° du produit (Fournisseur)

    pg00-05

    Fournisseur

    Rockland

    Specificité

    Protein G Alkaline Phosphatase Conjugated was prepared from chromatographically pure Protein G. Protein G Alkaline Phosphatase Conjugated assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Protein G. No reaction was observed against anti-Protein A.
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  • Indications d'application

    Protein G Alkaline Phosphatase Conjugated is suitable as a detection agent for primary antibodies that are of the IgG isotype. With the addition of pNPP in ELISA experiments, a yellow product is generated that can be detected on a plate reader.

    Commentaires

    Protein G Alkaline Phosphatase Conjugated is suitable as a detection agent for primary antibodies that are of the IgG isotype. With the addition of pNPP in ELISA experiments, a yellow product is generated that can be detected on a plate reader.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1.0 mg/mL

    Buffer

    0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C

    Date de péremption

    12 months
  • Antigène

    Protein G

    Sujet

    Protein G is a surface protein of two groups of Streptococcal bacteria that has the ability to bind immunoglobulins. Similar to Protein A, but with slightly different specificity, Protein G is an important agent in the purification of proteins due to its ability to bind the Fc region. Protein G also binds to albumin. Alkaline Phosphatase is an enzyme which removes phosphate groups from a variety of substate molecules. As the name implies, this enzyme functions best under basic pH. Alkaline Phosphatase can be utilized in molecular biology in DNA ligation experiments (keeping the DNA linear), radiolabeling preparations, and a detection mediator in ELISA experiments. Protein G Alkalline Phosphatase Conjugated is ideal for investigators involved in Signal Transduction, Molecular Biology, Cancer, and Cell Signaling.
    Synonyms: ProG, Streptococcus G protein, ALP, Alk Phos
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