Streptavidin Protein (HRP)

N° du produit ABIN964537

N° du produit (Fournisseur): s000-03

Protéine Native Streptavidin exprimée dans Streptomyces avidinii et a été mentionnée dans 4+ publications.

Aperçu rapide pour Streptavidin Protein (HRP) (ABIN964537)

Antigène: Streptavidin

Type de proteíne: Native

Origine: Streptomyces avidinii

Source: Streptomyces avidinii

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Dot Blot (DB), Immunofluorescence (IF), Immunoelectron Microscopy (IEM), Fluorescence Microscopy (FM)

Détail du produit

Purification/Conjugué: Cette Streptavidin protéine est marqué à la HRP.

N° du produit (Fournisseur): s000-03

Fournisseur: Rockland

Fonction: Streptavidin Peroxidase Conjugated

Purification: Streptavidin-HRP was prepared from chromatographically purified streptavidin.  Streptavidin Peroxidase conjugate was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Streptavidin.

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Streptavidin Peroxidase has been tested by dot blot and western blot and is a useful detection reagent for primary antibodies conjugated to biotin. Streptavidin Peroxidase can be utilized in Immunohistochemistry, Immunofluorescence, immuno-EM, Western Bl

Western Blot Dilution: 1:10,000 - 1:40,000

ELISA Dilution: 1:20,000 - 1:200,000

IF Microscopy Dilution: User Optimized

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent) (in 1.0 mL)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do NOT add Sodium Azide! Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Streptavidin Peroxidase Expiration date is one (1) year from date of receipt.

Date de péremption: 12 months

Référence

Metzner, Luckert, Lemuth, Hämmerle, Moos: "Towards an Electrochemical Immunosensor System with Temperature Control for Cytokine Detection." dans: Sensors (Basel, Switzerland), Vol. 18, Issue 5, (2018) (PubMed).

Vasek, Garber, Dorsey, Durrant, Bollman, Soung, Yu, Perez-Torres, Frouin, Wilton, Funk, DeMasters, Jiang, Bowen, Mennerick, Robinson, Garbow, Tyler, Suthar, Schmidt, Stevens, Klein: "A complement-microglial axis drives synapse loss during virus-induced memory impairment." dans: Nature, Vol. 534, Issue 7608, pp. 538-43, (2016) (PubMed).

Cantoni, Bollman, Licastro, Xie, Mikesell, Schmidt, Yuede, Galimberti, Olivecrona, Klein, Cross, Otero, Piccio: "TREM2 regulates microglial cell activation in response to demyelination in vivo." dans: Acta neuropathologica, Vol. 129, Issue 3, pp. 429-47, (2015) (PubMed).

Cowles, Li, Semmelhack, Cristea, Silhavy: "The free and bound forms of Lpp occupy distinct subcellular locations in Escherichia coli." dans: Molecular microbiology, Vol. 79, Issue 5, pp. 1168-81, (2011) (PubMed).

Détail du antigène

Antigène: Streptavidin

Sujet:

Synonyms: HRP-SA, Horseradish Peroxidase conjugated S avidin, Streptavidin HRP, Streptavidin conjugated to horseradish peroxidase (HRP), HRP-linked Streptavidin

Background: Streptavidin is isolated from bacteria, Streptomyces avidinii, and has an exceptionally high binding affinity for B7 (biotin). Rockland offers streptavidin in unconjugated and conjugated forms for common immunoassays including ELISA, western blotting, im

UniProt: P22629