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Streptavidin Protein (Alkaline Phosphatase (AP))

Cette protéine Recombinant Streptavidin est produite dans .
Rockland
N° du produit ABIN964538
N° du produit (Fournisseur): s000-05

Aperçu rapide pour Streptavidin Protein (Alkaline Phosphatase (AP)) (ABIN964538)

Antigène

Voir toutes Streptavidin Protéines
Streptavidin

Type de proteíne

Recombinant

Origine

  • 63
  • 2
  • 1
  • 1
  • 1
Streptomyces avidinii

Hôte

Veuillez nous consulter SVP

Application

Western Blotting (WB), ELISA
  • Purification/Conjugué

    Cette Streptavidin protéine est marqué à la Alkaline Phosphatase (AP).

    N° du produit (Fournisseur)

    s000-05

    Fournisseur

    Rockland

    Specificité

    Streptavidin Alkaline Phosphatase was prepared from electrophoretically pure Streptavidin. Streptavidin Alkaline Phosphatase was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Streptavidin. No reaction was observed against anti-Avidin.
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  • Indications d'application

    Streptavidin Alkaline Phosphatase is a useful detection reagent for primary antibodies conjugated to biotin. Streptavidin Peroxidase can be utilized in both Western Blotting and ELISA experiment formats in combination with the proper substrate (NPP-10).

    Commentaires

    Streptavidin Alkaline Phosphatase is a useful detection reagent for primary antibodies conjugated to biotin. Streptavidin Peroxidase can be utilized in both Western Blotting and ELISA experiment formats in combination with the proper substrate (NPP-10).

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1.0 mg/mL

    Buffer

    0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C

    Date de péremption

    12 months
  • Antigène

    Streptavidin

    Sujet

    Streptavidin is a a bacterial protein (from Streptomyces avidinii) that has an exceptionally high binding affinity for biotin (B7). Streptavidin-biotin binding is one of the strongest known non-covalent interactions and is highly resistant to many conditions that would typically cause dissociation (such as organic solvents, denaturants, detergents, and extreme temperatures or pH). Streptavidin's afinity for biotin can be employed in a variety of experimental uses, from purifications to standards, to means of detection or pull down experiments. Alkaline Phosphatase is an enzyme which removes phosphate groups from a variety of substate molecules. As the name implies, this enzyme functions best under basic pH. Alkaline Phosphatase can be utilized in molecular biology in DNA ligation experiments (keeping the DNA linear), radiolabeling preparations, and a detection mediator in ELISA experiments.
    Synonyms: ALP, Alk Phos, SA ALP

    UniProt

    P22629
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