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Streptavidin Protein (Alkaline Phosphatase (AP))

Cette protéine Native Streptavidin est produite dans Streptomyces avidinii.
Rockland
N° du produit ABIN964538
N° du produit (Fournisseur): s000-05

Aperçu rapide pour Streptavidin Protein (Alkaline Phosphatase (AP)) (ABIN964538)

Antigène

Voir toutes Streptavidin Protéines
Streptavidin

Type de proteíne

Native

Origine

  • 72
  • 2
  • 2
  • 1
  • 1
Streptomyces avidinii

Source

  • 50
  • 12
  • 2
  • 1
Streptomyces avidinii

Application

Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
  • Purification/Conjugué

    Cette Streptavidin protéine est marqué à la Alkaline Phosphatase (AP).

    N° du produit (Fournisseur)

    s000-05

    Fournisseur

    Rockland

    Fonction

    Streptavidin Alkaline Phosphatase Conjugated

    Purification

    Streptavidin-Alkaline Phosphatase was prepared from electrophoretically pure Streptavidin.   Alkaline Phosphatase conjugated Streptavidin was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf inte

    Stérilité

    Sterile filtered
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  • Indications d'application

    Immunohistochemistry Dilution: 1:200 - 1:1,000

    Application Note: Streptavidin Alkaline Phosphatase conjugated has been tested by dot blot and western blot and is a useful detection reagent for primary antibodies conjugated to biotin. Streptavidin Peroxidase can be utilized in both Western Blotting and ELISA experiment

    Western Blot Dilution: 1:1,000 - 1:4,000

    ELISA Dilution: 1:8,000 - 1:32,000

    Other: User Optimized

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1.0 mg/mL

    Buffer

    Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Conseil sur la manipulation

    DO NOT FREEZE. Dilute only prior to immediate use.

    Stock

    4 °C

    Stockage commentaire

    Store vial at 4° C before opening. DO NOT FREEZE. Streptavidin Alkaline Phosphatase conjugated is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss o Expiration date is one (1) year from date of receipt.

    Date de péremption

    12 months
  • Antigène

    Streptavidin

    Sujet

    Synonyms: SA alkaline phosphatase conjugate, S avidin conjugated to alkaline phosphatase, alkaline phosphatase conjugated to streptococcus avidin, streptavidin Alk Phos, SA-ALP, ALP conjugated streptavidin

    Background: Streptavidin is a bacterial protein (from Streptomyces avidinii) that has an exceptionally high binding affinity for biotin (B7). Streptavidin-biotin binding is one of the strongest known non-covalent interactions and is highly resistant to many conditio

    UniProt

    P22629
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