Streptavidin Protein (PE)

N° du produit ABIN964539

N° du produit (Fournisseur): s000-08

Cette protéine Streptavidin est exprimée dans .

Aperçu rapide pour Streptavidin Protein (PE) (ABIN964539)

Antigène: Streptavidin

Origine: Streptomyces avidinii

Hôte: Veuillez nous consulter SVP

Application: Flow Cytometry (FACS), Immunomicroscopy (IM)

Détail du produit

Purification/Conjugué: Cette Streptavidin protéine est marqué à la PE.

N° du produit (Fournisseur): s000-08

Fournisseur: Rockland

Specificité: Streptavidin Phycoerythrin was prepared from electrophoretically purified streptavidin isolated from Streptomyces avidinii conjugated to the chromophore R-Phycoerythrin. Free fluorochrome is removed by tandem molecular sieve chromatography.

Stérilité: Sterile filtered

Information d'application

Indications d'application: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of Streptavidin Phycoerythrin required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate.  Lesser amounts of Streptavidin Phycoerythrin may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

Commentaires:

Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of Streptavidin Phycoerythrin required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of Streptavidin Phycoerythrin may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 0.5 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: Streptavidin

Sujet: Streptavidin is a a bacterial protein (from Streptomyces avidinii) that has an exceptionally high binding affinity for biotin (B7). Streptavidin-biotin binding is one of the strongest known non-covalent interactions and is highly resistant to many conditions that would typically cause dissociation (such as organic solvents, denaturants, detergents, and extreme temperatures or pH). Streptavidin's afinity for biotin can be employed in a variety of experimental uses, from purifications to standards, to means of detection or pull down experiments. Phycoerythrin (PE) is a red-pigmented protein found in cyanobacteria and red algae. Phycoerythrin absorbs light blue-green/yellow light and emits lightly orange/yellow light.
Synonyms: Streptavidin PE, Phyco Streptavidin, SA Phycoerythrin, R-Phycoerythrin

UniProt: P22629