Protein A Protein (HRP)

N° du produit ABIN967967

Cette protéine Native Protein A est produite dans Staphylococcus aureus.

Aperçu rapide pour Protein A Protein (HRP) (ABIN967967)

Antigène: Protein A

Type de proteíne: Native

Origine: Staphylococcus aureus

Source: Staphylococcus aureus

Application: Western Blotting (WB)

Détail du produit

Purification/Conjugué: Cette Protein A protéine est marqué à la HRP.

Attributs du produit: Protein A has been reported to bind strongly to rabbit IgG and mouse IgG2 isotypes. However, protein A does not bind mouse IgM and weakly binds IgG1. When conjugated to agarose, protein A acts as a solid matrix facilitating the immunoprecipitation of antigen-antibody complexes. It can also be utilized for antibody purification. As a horseradish peroxidase conjugate, protein A can be used instead of secondary antibody detection methods in Western blotting with polyclonal antibodies and with IgG2a and IgG2b mouse monoclonal antibodies.
Horseradish peroxidase (HRP) was coupled to protein A at a concentration of 4 mg/mg of protein A using the periodate method. For the detection of antigen using polyclonal antibodies in Western blotting, a concentration of 0.5 µg/ml (1:2000 dilution) is suggested.

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Information d'application

Commentaires:

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 50 % glycerol, 20 mM sodium phosphate pH 7.5, 150 mM NaCl.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Détail du antigène

Antigène: Protein A

Autre désignation: ProteinA