CXCL16 Protéine

N° du produit ABIN987799

Protéine CXCL16 exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour CXCL16 Protéine (ABIN987799)

Antigène: CXCL16 (Chemokine (C-X-C Motif) Ligand 16 (CXCL16))

Activité biologique: Active

Origine: Souris

Source: Escherichia coli (E. coli)

Pureté: > 98 % by SDS-PAGE and HPLC analyses.

Détail du produit

Séquence: NQGSVAGSCS CDRTISSGTQ IPQGTLDHIR KYLKAFHRCP FFIRFQLQSK SVCGGSQDQW VRELVDCFER KECGTGHGKS FHHQKHL

Attributs du produit: Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay using murine lymphocytes is less than 1000 ng/ml, corresponding to a specific activity of >, 1.0 × 103 IU/mg.

niveau d'endotoxine: Level Less than 1EU/µg of rMuCXCL16 as determined by LAL method

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stock: 4 °C

Détail du antigène

Antigène: CXCL16 (Chemokine (C-X-C Motif) Ligand 16 (CXCL16))

Autre désignation: CXCL16

Sujet: Mouse CXCL16 (CXC chemokine 16) is a nonELR motifcontaining CXC chemokine with a transmembrane domain. CX3CL1/Fractalkine and CXCL16 are the only two transmembrane chemokines within the superfamily. Mouse CXCL16 cDNA encodes a 246 amino acid (aa) precursor protein with a putative 26 aa residue signal peptide,an 88 aa residue chemokine domain, an 87 aa residue mucinlike spacer region, a 22 aa residue transmembrane domain, and a 23 aa residue cytoplasmic tail. Mouse and human CXCL16 share 49% overall aa identity and 70% similarity in the chemokine domains. Mouse CXCL16 is produced by dendritic cells in lymphoid organ T cell zones and by cells in the splenic red pulp both as membranebound and soluble forms. Based on northern blot analysis,CXCL16 is also expressed in some nonlymphoid tissues such as lung, small intestine and kidney. The receptor for CXCL16 has been identified as CXCR6/Bonzo (STRL33 and TYMSTR), a receptor previously shown to be a coreceptor for HIV entry.CXCR6 is expressed on naive CD8 cells, naturalkiller T cells and activated CD8 and CD4 T cells. Synonym: CXCL16, Mouse. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in PBS.

Poids moléculaire: Recombinant murine CXCL16 is a 9.9 kDa protein containing 88 amino acid residues.