CX3CL1 Protéine

N° du produit ABIN987873

Cette protéine CX3CL1 est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour CX3CL1 Protéine (ABIN987873)

Antigène: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 97 % by SDS-PAGE and HPLC analyses.

Détail du produit

Séquence: QHHGVTKCNI TCSKMTSKIP VALLIHYQQN QASCGKRAII LETRQHRLFC ADPKEQWVKD AMQHLDRQAA ALTRN

Attributs du produit: Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay using human T-lymphocytes is less than 10 ng/ml, corresponding to a specific activity of >, 1.0 × 105 IU/mg.

niveau d'endotoxine: Level Less than 1EU/µg of rHuFractalkine/CX3CL1 as determined by LAL method

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stock: 4 °C

Détail du antigène

Antigène: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Autre désignation: Fractalkine/CX3CL1

Sujet: Fractalkine, also named neurotactin, is a novel chemokine recently identified through bioinformatics. Fractalkine has a unique C-X3-C cysteine motif near the amino-terminus and is the first member of a fourth branch of the chemokine superfamily. Unlike other known chemokines, fractalkine is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. Human fractalkine cDNA encodes a 397 amino acid (aa) residue membrane protein with a 24 aa residue predicted signal peptide, a 76 aa residue chemokine domain, a 241 aa residue stalk region containing 17 degenerate mucin-like repeats, a 19 aa residue transmembrane segment and a 37 aa residue cytoplasmic domain. The extracellular domain of human fractalkine can be released, possibly by proteolysis at the dibasic cleavage site proximal to the membrane, to generate soluble fractalkine. The soluble chemokine domain of human fractalkine was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse fractalkine was reported to chemoattract neutrophils and T-lymphocytes but not monocytes. Synonym: Fractalkine/CX3CL1, Human. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 7.4, 50mM NaCl.

Poids moléculaire: 8.5 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids and comprises only the chemokine domain of Human Fractalkine.

Pathways: Synaptic Membrane