RGL2 anticorps
Aperçu rapide pour RGL2 anticorps (ABIN4966294)
Antigène
Reactivité
Hôte
Clonalité
Application
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Fonction
- Anti-RGL2 | RGA-like protein 2 (DELLA protein)
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Réactivité croisée (Details)
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Arabidopsis thaliana
Not reactive in: Brassica napus, Populus sp., Triticum sp. -
Purification
- Immunogen affinity purified serum
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Immunogène
- KLH-conjugated synthetic peptide derived from Arabidopsis thaliana RGL2 protein
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Indications d'application
- 1 : 1000 (WB), transfected Arabidopsis thaliana protoplasts
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Commentaires
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As this protein is expressed in tissue in very low levels, please load at least 50 μg/well.Recommended extraction and denaturation protocol: Arabidopsis thaliana seedlings were ground in liquid nitrogen (100 μl of 2.5 x Laemli for 80-120 mg of homogenized material) and boiled for 2 min. at 95°C in 2,5x Laemmli Buffer (with 60 Mm DTT final concentration) otherwise RGA protein will degrade.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 50 μL of sterile water
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Buffer
- Immunogen affinity purified serum in PBS pH 7.4.
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Conseil sur la manipulation
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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Stock
- -20 °C
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Stockage commentaire
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- RGL2 (DELLA Protein RGL2 (RGL2))
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Autre désignation
- RGA-like protein 2 (DELLA protein) (RGL2)
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Sujet
- DELLA protein RGL2 is a probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Involved in the biosynthesis of abscisic acid (ABA), delays flowering and leaf production, reulates floral development, petal, stamen and anther development. Predominantly expressed in germinating seeds, flowers and siliques. Synonymes: GRAS family protein 15, AtGRAS-15, Scarecrow-like protein 19, AtSCL19.
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Poids moléculaire
- 60 kDa
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ID gène
- 821251
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UniProt
- Q8GXW1
Antigène
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