Selenoprotein F Protein (SELENOF) (AA 29-165) (His tag)
Cette protéine Recombinant Selenoprotein F est exprimée dans Escherichia coli (E. coli).
N° du produit ABIN7676652
Aperçu rapide pour Selenoprotein F Protein (SELENOF) (AA 29-165) (His tag) (ABIN7676652)
Antigène
Selenoprotein F (SELENOF)
Type de proteíne
Recombinant
Origine
Humain
Source
Escherichia coli (E. coli)
Application
SDS-PAGE (SDS)
Pureté
> 95 %
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Attributs du protein
- AA 29-165
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Purification/Conjugué
- Cette Selenoprotein F protéine est marqué à la His tag.
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Séquence
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VSAFGAE FSSEACRELG FSSNLLCSSC DLLGQFNLLQ LDPDCRGCCQ EEAQFETKKL YAGAILEVCG CKLGRFPQVQ AFVRSDKPKL FRGLQIKYVR GSDPVLKLLD DNGNIAEELS ILKWNTDSVE EFLSEKLERI -
Purification
- > 95% by SDS-PAGE
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1 mM DTT
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Stock
- 4 °C,-20 °C,-80 °C
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Stockage commentaire
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- Selenoprotein F (SELENOF)
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Autre désignation
- Seleno F
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Sujet
- SEP15 is a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the uGA codon that normally signals translation termination. The 3' uTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of uGA as a Sec codon rather than as a stop signal. Studies in mouse suggest that this selenoprotein may have redox function and may be involved in the quality control of protein folding. This gene is localized on chromosome 1p31, a genetic locus commonly mutated or deleted in human cancers. Recombinant human SEP15 (SC96C) protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Poids moléculaire
- 17.7 kDa (160aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_004252
Antigène
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