Chèvre anti-Humain IgA, IgG, IgM Anticorps (HRP)

N° du produit ABIN100792

N° du produit (Fournisseur): 609-103-130

Aperçu rapide pour Chèvre anti-Humain IgA, IgG, IgM Anticorps (HRP) (ABIN100792)

Antigène: IgA, IgG, IgM

Clonalité: Polyclonal

Conjugué: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP)

Isotype: IgG

Détail du produit

Reactivité: Humain

Hôte: Chèvre

N° du produit (Fournisseur): 609-103-130

Fournisseur: Rockland

Fonction: Human IgG IgA IgM (H&L) Antibody Peroxidase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgG, Human IgA and Human IgM.  This reagent is suitable for the detection of all Human immunoglobulin subclasses and chain combinations.

Attributs du produit: Anti-Human IgG (H&L) Peroxidase generated in goat detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present.

Purification: This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads.

Immunogène:

Immunogen: Anti-Human IgG IgA IgM (H&L) was produced by repeated immunization with Human IgG, IgA and IgM whole molecules in goat.

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: Anti-Human IgG IgA IgM (H&L) Peroxidase Antibody has been tested by ELISA and western blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 Immunoprecipitation Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Référence

Jin, Nesbitt, Yang, Chen, Horowitz, Jones, Vandergaast, Carey, Reiter, Russell, Kyratsous, Hooper, Hamilton, Ferreira, Deng, Straus, Baras, Hillyer, Luchsinger: "Seroprevalence of anti-SARS-CoV-2 antibodies in a cohort of New York City metro blood donors using multiple SARS-CoV-2 serological assays: Implications for controlling the epidemic and "Reopening"." dans: PloS one, Vol. 16, Issue 4, pp. e0250319, (2021) (PubMed).

Nesbitt, Jin, Hogan, Yang, Chen, Chan, Simon, Vargas, King, Huard, Bandy, Hillyer, Luchsinger: "Low Seroprevalence of SARS-CoV-2 in Rhode Island blood donors during may 2020 as determined using multiple serological assay formats." dans: BMC infectious diseases, Vol. 21, Issue 1, pp. 871, (2021) (PubMed).

Luchsinger, Ransegnola, Jin, Muecksch, Weisblum, Bao, George, Rodriguez, Tricoche, Schmidt, Gao, Jawahar, Pal, Schnall, Zhang, Strauss, Yazdanbakhsh, Hillyer, Bieniasz, Hatziioannou: "Serological Assays Estimate Highly Variable SARS-CoV-2 Neutralizing Antibody Activity in Recovered COVID19 Patients." dans: Journal of clinical microbiology, (2020) (PubMed).

Crawford, Tempel, Streblow, Kreklywich, Smith, Picker, Nelson, Caposio: "Human Cytomegalovirus Induces Cellular and Humoral Virus-specific Immune Responses in Humanized BLT Mice." dans: Scientific reports, Vol. 7, Issue 1, pp. 937, (2018) (PubMed).

Détail du antigène

Antigène: IgA, IgG, IgM

Autre désignation: IgG + IgA + IgM

Classe de substances: Antibody

Sujet: Anti-Human IgG IgA IgM (H&L) Peroxidase Antibody generated in goat detects human (heavy and light chain) immunoglobulin G, A, and M. Immunoglobulin G binds to antigens and can neutralize or opsonize targets, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunoglobulin molecules linked through their F(c) domain by the J chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.