Chèvre anti-Souris IgA, IgG, IgM Anticorps (Biotin) - Preadsorbed

N° du produit ABIN100812

N° du produit (Fournisseur): 610-106-430

Aperçu rapide pour Chèvre anti-Souris IgA, IgG, IgM Anticorps (Biotin) - Preadsorbed (ABIN100812)

Antigène: IgA, IgG, IgM

Clonalité: Polyclonal

Conjugué: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Détail du produit

Reactivité: Souris

Hôte: Chèvre

N° du produit (Fournisseur): 610-106-430

Fournisseur: Rockland

Fonction: Mouse IgG IgA IgM (H&L) Antibody Biotin Conjugated Pre-Adsorbed

Réactivité croisée (Details): Minimal crossreactivity against Bv, Hs, Hu Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Mouse IgG, Mouse IgA and Mouse IgM.  No reaction was observed against bovine, horse or human serum proteins.  This reagent is suitable for the detection of all mouse isotypes and chain combinations.  ELISA was used to confirm specificity at less than 1 % of the target signal.

Attributs du produit: Anti-Mouse IgG IgA IgM Biotin Antibody generated in goat detects reactivity to Mouse IgG, Mouse IgA, and Mouse IgM.

Purification: This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by cross adsorption to remove unwanted specificities.

Immunogène: Optional[Immunogen]: Mouse IgG IgA and IgM whole molecule

Information d'application

Indications d'application: Application Note: Anti-Mouse IgG IgA IgM Biotin Antibody has been tested by ELISA and is useful for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,500 - 1:15,000 Western Blot Dilution: 1:3,000 - 1:30,000 ELISA Dilution: 1:300,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détail du antigène

Antigène: IgA, IgG, IgM

Autre désignation: IgG,IgA,IgM

Classe de substances: Antibody

Sujet: Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.