Lapin anti-Poulet IgG (F(ab')2 Region) Anticorps (Alkaline Phosphatase (AP))
Aperçu rapide pour Lapin anti-Poulet IgG (F(ab')2 Region) Anticorps (Alkaline Phosphatase (AP)) (ABIN101060)
Antigène
Clonalité
Conjugué
Application
Isotype
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Épitope
- F(ab')2 Region
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Reactivité
- Poulet
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Hôte
- Lapin
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N° du produit (Fournisseur)
- 603-4504
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Fournisseur
- Rockland
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Fonction
- Chicken IgG F(ab')2 Antibody Alkaline Phosphatase Conjugated
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Réactivité croisée (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Chicken IgG, Chicken IgG F(ab')2 and Chicken Serum. No reaction was observed against Chicken IgG F(c).
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Attributs du produit
- Anti-Chicken IgG F(c) Antibody detects specifically Chicken IgG F(c). It is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH .
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Purification
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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Stérilité
- Sterile filtered
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Immunogène
- Optional[Immunogen]: Chicken IgG F(ab')2 fragment
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Indications d'application
- Application Note: Anti-Chicken IgG F(ab')2 Alkaline Phosphatase antibody has been tested by ELISA and dot blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:3,500 Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide -
Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C
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Stockage commentaire
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
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Date de péremption
- 12 months
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- IgG
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Classe de substances
- Antibody
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Sujet
- Anti-Chicken IgG F(ab')2 Antibody generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind Chicken IgG F(c) will not bind Chicken IgG F(ab')2 Molecules.
Antigène
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