Âne anti-Chévre IgG (Heavy & Light Chain) Anticorps - Preadsorbed

N° du produit ABIN101178

N° du produit (Fournisseur): 605-701-125

Aperçu rapide pour Âne anti-Chévre IgG (Heavy & Light Chain) Anticorps - Preadsorbed (ABIN101178)

Antigène: IgG

Clonalité: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Détail du produit

Épitope: Heavy & Light Chain

Reactivité: Chévre

Hôte: Âne

N° du produit (Fournisseur): 605-701-125

Fournisseur: Rockland

Fonction: Goat IgG (H&L) Antibody Pre-Adsorbed

Réactivité croisée (Details): Minimal crossreactivity against Ch GP Ham Hs Ms Rb & Rt Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Goat IgG and Goat Serum.  No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit or Rat Serum Proteins.  Specificity was confirmed using ELISA minimal cross reactivity against the targets stated above.

Attributs du produit: Anti-Goat IgG Antibody generated in donkey detects goat IgG.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Stérilité: Sterile filtered

Immunogène: Optional[Immunogen]: Goat IgG whole molecule

Information d'application

Indications d'application: Application Note: Anti-Goat IgG antibody has been tested by ELISA and is suitable for western blot and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:150,000 - 1:250,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.25 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Date de péremption: 12 months

Référence

Jain, Liu, Xiang, Ha: "Single-molecule pull-down for studying protein interactions." dans: Nature protocols, Vol. 7, Issue 3, pp. 445-52, (2012) (PubMed).

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.