Lapin anti-Chévre IgG (F(ab')2 Region) Anticorps (TRITC) - Preadsorbed
Aperçu rapide pour Lapin anti-Chévre IgG (F(ab')2 Region) Anticorps (TRITC) - Preadsorbed (ABIN101242)
Antigène
Clonalité
Conjugué
Application
Isotype
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Épitope
- F(ab')2 Region
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Reactivité
- Chévre
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Hôte
- Lapin
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N° du produit (Fournisseur)
- 605-4015
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Fournisseur
- Rockland
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Fonction
- Goat IgG F(ab')2 Antibody Rhodamine Conjugated Pre-Adsorbed
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Réactivité croisée (Details)
- Minimal crossreactivity against Human Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG, Goat IgG F(ab')2 and Goat Serum. No reaction was observed against Goat IgG F(c) or Human Serum Proteins.
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Attributs du produit
- Anti-Goat IgG Rhodamine Antibody generated in rabbit detects goat IgG.
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Purification
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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Immunogène
- Optional[Immunogen]: Goat IgG F(ab')2 fragment
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Indications d'application
- Application Note: Anti-Goat IgG F(ab')2 Rhodamine Antibody has been tested by dot blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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Concentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide -
Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Date de péremption
- 12 months
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- IgG
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Classe de substances
- Antibody
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Sujet
- Anti-Goat IgG F(ab')2 Rhodamine Antibody generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind goat IgG F(c) will not bind goat IgG F(ab')2 Molecules.
Antigène
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