Chèvre anti-Cobaye IgG (Fc Region) Anticorps (Alkaline Phosphatase (AP))

N° du produit ABIN101327

N° du produit (Fournisseur): 606-1503

Aperçu rapide pour Chèvre anti-Cobaye IgG (Fc Region) Anticorps (Alkaline Phosphatase (AP)) (ABIN101327)

Antigène: IgG

Clonalité: Polyclonal

Conjugué: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)

Isotype: IgG

Détail du produit

Épitope: Fc Region

Reactivité: Cobaye

Hôte: Chèvre

N° du produit (Fournisseur): 606-1503

Fournisseur: Rockland

Fonction: Guinea Pig IgG Fc Antibody Alkaline Phosphatase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Guinea Pig IgG, Guinea Pig IgG F(c) and Guinea Pig Serum.  No reaction was observed against Guinea Pig IgG F(ab).

Attributs du produit: Anti-Guinea Pig IgG Alkaline Phosphatase Antibody generated in goat detects guinea pig IgG.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Stérilité: Sterile filtered

Immunogène: Optional[Immunogen]: Guinea Pig IgG F(c) fragment

Information d'application

Indications d'application: Application Note: Anti-Guinea Pig IgG F(c) Alkaline Phosphatase has been tested by ELISA and dot blot. This product has been assayed against 1.0 μg of Guinea Pig IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:5,000 is suggested for this product.  Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Receptors bind the Fc portion of Guinea Pig IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.