Lapin anti-Cobaye IgG (Fc Region) Anticorps (Biotin)

N° du produit ABIN101335

N° du produit (Fournisseur): 606-4603

Aperçu rapide pour Lapin anti-Cobaye IgG (Fc Region) Anticorps (Biotin) (ABIN101335)

Antigène: IgG

Clonalité: Polyclonal

Conjugué: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Détail du produit

Épitope: Fc Region

Reactivité: Cobaye

Hôte: Lapin

N° du produit (Fournisseur): 606-4603

Fournisseur: Rockland

Fonction: Guinea Pig IgG Fc Antibody Biotin Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Guinea Pig IgG, Guinea Pig IgG F(c) and Guinea Pig Serum.  No reaction was observed against Guinea Pig IgG F(ab).

Attributs du produit: Anti-Guinea Pig IgG Biotin Antibody generated in rabbit detects guinea pig IgG.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogène: Optional[Immunogen]: Guinea Pig IgG F(c) fragment

Information d'application

Indications d'application: Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. This product has been assayed against 1.0 μg of Guinea Pig IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Receptors bind the Fc portion of guinea pig IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.