Lapin anti-Hamsters dorés syriens IgG (F(ab')2 Region) Anticorps
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- Antigène Tous les produits IgG
- IgG
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Épitope
- F(ab')2 Region
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Reactivité
- Hamsters dorés syriens
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Specificité
- IgG F(ab')2
- Attributs du produit
- Concentration Definition: by UV absorbance at 280 nm
- Purification
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Hamster IgG, Hamster IgG F(ab')2 and Hamster Serum. No reaction was observed against Hamster IgG F(c).
- Immunogène
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Immunogen: Hamster IgG F(ab')2 fragment
- Isotype
- IgG
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- Indications d'application
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Anti-Golden Syrian Hamster IgG F(ab')2 antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
ELISA Dilution: 1:20,000 - 1:100,000
Western Blot Dilution: 1:2,000 - 1:10,000
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 5.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 10.0 mg/mL
- Buffer
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- RT,4 °C,-20 °C
- Date de péremption
- 12 months
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- Antigène
- IgG
- Abstract
- IgG Produits
- Classe de substances
- Antibody
- Sujet
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Synonyms: rabbit anti-Golden Syrian Hamster IgG F(ab')2 fragment Antibody, rabbit anti-Golden Syrian Hamster IgG Fab2 fragment Antibody, rabbit anti Golden Syrian Hamster IgG Fab2
Background: Anti-Golden Syrian Hamster IgG F(ab')2 Antibody generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind Golden Syrian Hamster IgG F(c) will not bind Golden Syrian Hamster IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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