Lapin anti-Hamsters dorés syriens IgG (F(ab')2 Region) Anticorps (Alkaline Phosphatase (AP))

N° du produit ABIN101391

N° du produit (Fournisseur): 607-4504

Aperçu rapide pour Lapin anti-Hamsters dorés syriens IgG (F(ab')2 Region) Anticorps (Alkaline Phosphatase (AP)) (ABIN101391)

Antigène: IgG

Clonalité: Polyclonal

Conjugué: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Détail du produit

Épitope: F(ab')2 Region

Reactivité: Hamsters dorés syriens

Hôte: Lapin

N° du produit (Fournisseur): 607-4504

Fournisseur: Rockland

Fonction: Golden Syrian Hamster IgG F(ab')2 Antibody Alkaline Phosphatase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Hamster IgG and Hamster Serum.   No reaction was observed against Hamster IgG F(c).

Attributs du produit: Anti-Golden Syrian Hamster IgG Alkaline Phosphatase Antibody generated in rabbit detects Golden Syrian Hamster IgG.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Hamster IgG coupled to agarose.

Stérilité: Sterile filtered

Immunogène: Optional[Immunogen]: Hamster IgG F(ab')2 fragment

Information d'application

Indications d'application: Application Note: This product has been assayed against 1.0 μg of Hamster IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.  Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.8 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Anti-Golden Syrian Hamster IgG F(ab')2 Alkaline Phosphatase Antibody generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind Golden Syrian Hamster IgG F(c) will not bind Golden Syrian Hamster IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.