Aperçu rapide pour Chèvre anti-Cheval IgG (Heavy & Light Chain) Anticorps (ABIN101401)
Antigène
IgG
Clonalité
Polyclonal
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Isotype
IgG
Épitope
Heavy & Light Chain
Reactivité
Cheval
Hôte
Chèvre
N° du produit (Fournisseur)
608-1102
Fournisseur
Rockland
Fonction
Horse IgG (H&L) Antibody
Réactivité croisée (Details)
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Horse IgG and Horse Serum.
Attributs du produit
Anti-Horse IgG F(ab')2 Rhodamine Antibody generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind horse IgG F(c) will not bind horse IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose.
Application Note: Anti-Horse IgG antibody has been tested by ELISA and is suitable for western blot and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized
Restrictions
For Research Use only
Format
Liquid
Concentration
10 mg/mL
Buffer
Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stock
4 °C,-20 °C
Stockage commentaire
Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Date de péremption
12 months
Antigène
IgG
Classe de substances
Antibody
Sujet
Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.