Aperçu rapide pour Chèvre anti-Cheval IgG (Fc Region) Anticorps (Alkaline Phosphatase (AP)) (ABIN101445)
Antigène
IgG
Clonalité
Polyclonal
Conjugué
Alkaline Phosphatase (AP)
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Isotype
IgG
Épitope
Fc Region
Reactivité
Cheval
Hôte
Chèvre
N° du produit (Fournisseur)
608-1503
Fournisseur
Rockland
Fonction
Horse IgG Fc Antibody Alkaline Phosphatase Conjugated
Réactivité croisée (Details)
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Horse IgG, Horse IgG F(c) and Horse Serum. No reaction was observed against Horse IgG F(ab).
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Application Note: Anti-Horse IgG F(c) Alkaline Phosphatase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized
Restrictions
For Research Use only
Format
Liquid
Concentration
1.0 mg/mL
Buffer
Optional[Buffer]: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stock
4 °C
Stockage commentaire
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Date de péremption
12 months
Antigène
IgG
Classe de substances
Antibody
Sujet
Receptors bind the Fc portion of horse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.