Aperçu rapide pour Chèvre anti-Cheval IgG (Fc Region) Anticorps (HRP) (ABIN101448)
Antigène
IgG
Clonalité
Polyclonal
Conjugué
HRP
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Isotype
IgG
Épitope
Fc Region
Reactivité
Cheval
Hôte
Chèvre
N° du produit (Fournisseur)
608-1303
Fournisseur
Rockland
Fonction
Horse IgG Fc Antibody Peroxidase Conjugated
Réactivité croisée (Details)
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Horse IgG, Horse IgG F(c) and Horse Serum. No reaction was observed against Horse IgG F(ab).
Attributs du produit
Anti-Horse IgG Peroxidase Antibody generated in goat detects horse IgG.
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Application Note: Anti-Horse IgG F(c) Peroxidase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:10,000 ELISA Dilution: 1:10,000 - 1:50,000 Other: User Optimized
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
Concentration
2.0 mg/mL
Buffer
Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Agent conservateur
Gentamicin sulfate
Précaution d'utilisation
This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Date de péremption
12 months
Antigène
IgG
Classe de substances
Antibody
Sujet
Receptors bind the Fc portion of horse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.