Chèvre anti-Humain IgG (Heavy & Light Chain) Anticorps

N° du produit ABIN101509

N° du produit (Fournisseur): 209-1102

Aperçu rapide pour Chèvre anti-Humain IgG (Heavy & Light Chain) Anticorps (ABIN101509)

Antigène: IgG

Clonalité: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Détail du produit

Épitope: Heavy & Light Chain

Reactivité: Humain

Hôte: Chèvre

N° du produit (Fournisseur): 209-1102

Fournisseur: Rockland

Fonction: Human IgG (H&L) Antibody

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG and Human Serum.

Purification: Anti-HUMAN IgG (H&L) (GOAT) antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogène: Human IgG whole molecule

Information d'application

Indications d'application: Application Note: Suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency. Specific conditions should be optimized by researcher. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 5.0 mL

Concentration: 10 mg/mL

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Date de péremption: 12 months

Référence

Bobo, Phelan, Rebhahn, Piepenbrink, Zheng, Mosmann, Kobie, DeLouise: "Microbubble array diffusion assay for the detection of cell secreted factors." dans: Lab on a chip, Vol. 14, Issue 18, pp. 3640-50, (2015) (PubMed).

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.