Chèvre anti-Humain IgG (Heavy & Light Chain) Anticorps (FITC) - Preadsorbed
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- Antigène Tous les produits IgG
- IgG
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Épitope
- Heavy & Light Chain
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Reactivité
- Humain
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Hôte
- Chèvre
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Clonalité
- Polyclonal
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Conjugué
- FITC
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Application
- Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- Attributs du produit
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FITC Anti-Human IgG Secondary Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Synonyms: FITC Conjugated Secondary Antibodies, anti-human IgG FITC - Purification
- Preadsorption: Solid phase absorption
- Immunogène
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Immunogen: Human IgG whole molecule
- Isotype
- IgG
- Labeling Ratio
- 3.4
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- Indications d'application
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Application Note: Secondary antibodies aid in the detection or sorting of target antigens by binding to the primary antibody. Anti-Human IgG (H&L) Fluorescein antibody is ideal for Flow Cytometry, Immunofluorescence Microscopy as well as other antibody detection methods where highly cross adsorbed (Pre Adsorbed) methods are required.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
IF Microscopy Dilution: 1:1,000 - 1:5,000
- Commentaires
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Excitation/Emission wavelength: 494 nm/514 nm
Concentration (detection method): 1.0 mg/mL (by UV absorbance at 280 nm) - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Product is photosensitive and should be protected from light.
- Stock
- RT,4 °C,-20 °C
- Date de péremption
- 12 months
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- Antigène
- IgG
- Abstract
- IgG Produits
- Classe de substances
- Antibody
- Sujet
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Synonyms: goat anti-Human IgG fluorescein conjugated Antibody, goat anti-Human IgG Antibody FITC conjugation
Background: Anti-Human IgG (H&L) Fluorescein generated in goat detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present. It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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