Âne anti-Souris IgG (Heavy & Light Chain) Anticorps (HRP) - Preadsorbed

N° du produit ABIN101709

N° du produit (Fournisseur): 610-703-124

Aperçu rapide pour Âne anti-Souris IgG (Heavy & Light Chain) Anticorps (HRP) - Preadsorbed (ABIN101709)

Antigène: IgG

Clonalité: Polyclonal

Conjugué: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Détail du produit

Épitope: Heavy & Light Chain

Reactivité: Souris

Hôte: Âne

N° du produit (Fournisseur): 610-703-124

Fournisseur: Rockland

Fonction: Mouse IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Réactivité croisée (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Mouse IgG and Mouse Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.

Attributs du produit: Anti-Mouse IgG (H&L) peroxidase conjugated antibody generated in donkey detects specifically mouse IgG (H&L).

Purification: Mouse IgG (H&L) Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogène:

Immunogen: Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in donkey.

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: Anti-Mouse IgG Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other peroxidase antibody-based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:20,000 ELISA Dilution: 1:20,000 - 1:80,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Référence

Czulkies, Mastroianni, Lutz, Lang, Schwan, Schmidt, Lassmann, Zeiser, Aktories, Papatheodorou: "Loss of LSR affects epithelial barrier integrity and tumor xenograft growth of CaCo-2 cells." dans: Oncotarget, Vol. 8, Issue 23, pp. 37009-37022, (2018) (PubMed).

Schorch, Heni, Zahaf, Brummer, Mione, Schmidt, Papatheodorou, Aktories: "Targeting oncogenic Ras by the Clostridium perfringens toxin TpeL." dans: Oncotarget, Vol. 9, Issue 23, pp. 16489-16500, (2018) (PubMed).

Klaric, Thomas, Dottori, Leong, Koblar, Lewis: "A reduction in Npas4 expression results in delayed neural differentiation of mouse embryonic stem cells." dans: Stem cell research & therapy, Vol. 5, Issue 3, pp. 64, (2015) (PubMed).

Hemmasi, Czulkies, Schorch, Veit, Aktories, Papatheodorou: "Interaction of the Clostridium difficile Binary Toxin CDT and Its Host Cell Receptor, Lipolysis-stimulated Lipoprotein Receptor (LSR)." dans: The Journal of biological chemistry, Vol. 290, Issue 22, pp. 14031-44, (2015) (PubMed).

Litjens, Nguyen, Castro, Aromataris, Jones, Barritt, Rychkov: "Phospholipase C-gamma1 is required for the activation of store-operated Ca2+ channels in liver cells." dans: The Biochemical journal, Vol. 405, Issue 2, pp. 269-76, (2007) (PubMed).

Dazy, Damiola, Parisey, Beug, Gandrillon: "The MEK-1/ERKs signalling pathway is differentially involved in the self-renewal of early and late avian erythroid progenitor cells." dans: Oncogene, Vol. 22, Issue 58, pp. 9205-16, (2004) (PubMed).

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Anti-Mouse IgG whole molecule antibody generated in donkey detects specifically Mouse IgG whole molecule . This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.