Lapin anti-Souris IgG (Heavy & Light Chain) Anticorps (HRP)

N° du produit ABIN101781

N° du produit (Fournisseur): 210-4302

Aperçu rapide pour Lapin anti-Souris IgG (Heavy & Light Chain) Anticorps (HRP) (ABIN101781)

Antigène: IgG

Clonalité: Polyclonal

Conjugué: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Détail du produit

Épitope: Heavy & Light Chain

Reactivité: Souris

Hôte: Lapin

N° du produit (Fournisseur): 210-4302

Fournisseur: Rockland

Fonction: Mouse IgG (H&L) Antibody Peroxidase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum.

Attributs du produit: Anti-Mouse IgM fluorescein antibody specifically detects mouse IgM.

Purification: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogène: Optional[Immunogen]: Mouse IgG whole molecule

Information d'application

Indications d'application: Application Note: This product is designed for ELISA and western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:10,000 ELISA Dilution: 1:10,000 - 1:50,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL

Concentration: 10.0 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:None

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Référence

Gullicksen, Hausman, Dean, Hartzell, Baile: "Adipose tissue cellularity and apoptosis after intracerebroventricular injections of leptin and 21 days of recovery in rats." dans: International journal of obesity and related metabolic disorders : journal of the International Association for the Study of Obesity, Vol. 27, Issue 3, pp. 302-12, (2003) (PubMed).

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.