Lapin anti-Souris IgG (Fc Region) Anticorps (Alkaline Phosphatase (AP))
Aperçu rapide pour Lapin anti-Souris IgG (Fc Region) Anticorps (Alkaline Phosphatase (AP)) (ABIN101868)
Antigène
Clonalité
Conjugué
Application
Isotype
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Épitope
- Fc Region
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Reactivité
- Souris
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Hôte
- Lapin
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N° du produit (Fournisseur)
- 610-4503
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Fournisseur
- Rockland
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Fonction
- Mouse IgG Fc Antibody Alkaline Phosphatase Conjugated
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Réactivité croisée (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against Mouse IgG F(ab).
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Attributs du produit
- Anti-Mouse IgG Alkaline Phosphatase antibody generated in rabbit detects reactivity to Mouse IgG.
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Purification
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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Stérilité
- Sterile filtered
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Immunogène
- Optional[Immunogen]: Mouse IgG F(c) fragment
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Indications d'application
- Application Note: Anti-Mouse IgG F(c) Alkaline Phosphatase conjugate has been tested by dot blot and ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:5,000 - 1:15,000 Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide -
Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C
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Stockage commentaire
- Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
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Date de péremption
- 12 months
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- IgG
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Classe de substances
- Antibody
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Sujet
- Receptors bind the Fc portion of mouse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.
Antigène
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