Chèvre anti-Porc IgG (Heavy & Light Chain) Anticorps (FITC)

N° du produit ABIN101878

N° du produit (Fournisseur): 614-1202

Aperçu rapide pour Chèvre anti-Porc IgG (Heavy & Light Chain) Anticorps (FITC) (ABIN101878)

Antigène: IgG

Clonalité: Polyclonal

Conjugué: FITC

Application: Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Isotype: IgG

Détail du produit

Épitope: Heavy & Light Chain

Reactivité: Porc

Hôte: Chèvre

N° du produit (Fournisseur): 614-1202

Fournisseur: Rockland

Fonction: Swine IgG (H&L) Antibody Fluorescein Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Swine IgG and Swine Serum.

Attributs du produit: Anti-Swine IgG secondary antibody generated in goat detects specifically swine IgG. This secondary antibody anti-Swine is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads.

Immunogène: Optional[Immunogen]: Swine IgG whole molecule

Labeling Ratio: 2.33

Information d'application

Indications d'application: Application Note: Anti-Swine IgG (H&L) Fluorescein Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Swine IgG Antibody is conjugated to Fluorescein.