Chèvre anti-Porc IgG (Heavy & Light Chain) Anticorps (HRP)
Aperçu rapide pour Chèvre anti-Porc IgG (Heavy & Light Chain) Anticorps (HRP) (ABIN101879)
Antigène
Clonalité
Conjugué
Application
Isotype
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Épitope
- Heavy & Light Chain
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Reactivité
- Porc
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Hôte
- Chèvre
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N° du produit (Fournisseur)
- 614-1302
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Fournisseur
- Rockland
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Fonction
- Swine IgG (H&L) Antibody Peroxidase Conjugated
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Réactivité croisée (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Swine IgG and Swine Serum.
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Attributs du produit
- Anti-Swine IgG (H&L) Fluorescein Antibody generated in goat detects reactivity to swine IgG.
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Purification
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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Immunogène
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Immunogen: Anti-Swine IgG whole molecule was produced by repeated immunization with Swine IgG whole molecule in goat.
Immunogen Type: Native Protein
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Indications d'application
- Application Note: Anti-Swine IgG Peroxidase conjugated has been tested by ELISA and western blot and is assayed against 1.0 μg of Swine IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:10,000 ELISA Dilution: 1:100,000 - 1:200,000 Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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Concentration
- 2.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
Agent conservateur
- Gentamicin sulfate
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Précaution d'utilisation
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Date de péremption
- 12 months
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- IgG
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Classe de substances
- Antibody
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Sujet
- Anti-Swine IgG whole molecule antibody generated in goat detects specifically Swine IgG whole molecule. This secondary antibody anti-Rat is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Antigène
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