Chèvre anti-Rat IgG (Heavy & Light Chain) Anticorps (HRP) - Preadsorbed

N° du produit ABIN102145

N° du produit (Fournisseur): 612-103-120

Aperçu rapide pour Chèvre anti-Rat IgG (Heavy & Light Chain) Anticorps (HRP) - Preadsorbed (ABIN102145)

Antigène: IgG

Clonalité: Polyclonal

Conjugué: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Détail du produit

Épitope: Heavy & Light Chain

Reactivité: Rat

Hôte: Chèvre

N° du produit (Fournisseur): 612-103-120

Fournisseur: Rockland

Fonction: Rat IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Réactivité croisée (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rat IgG and Rat Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.

Attributs du produit: Anti-Rat IgG (H&L) rhodamine Antibody generated in goat detects reactivity to Rat IgG.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogène:

Immunogen: Anti-Rat IgG whole molecule was produced by repeated immunization with Rat IgG whole molecule in goat.

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: Anti-Rat IgG Peroxidase Conjugated Antibody has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:500 - 1:1,500 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:70,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Référence

Mousa, Alhumaydhi, Abdellatif, Abdulmonem, AlKhowailed, Alsagaby, Al Rugaie, Alnuqaydan, Aljohani, Aljasir, Alwashmi, Soliman, Yosof, Elsheikh, Babiker, Alsuhaibani, Hegazy, Seleem: "Curcumin and ustekinumab cotherapy alleviates induced psoriasis in rats through their antioxidant, anti-inflammatory, and antiproliferative effects." dans: Cutaneous and ocular toxicology, pp. 1-10, (2021) (PubMed).

Schmitt, Funk, Blum, Asan, Andersen, Rülicke, Sendtner, Buchner: "Initial characterization of a Syap1 knock-out mouse and distribution of Syap1 in mouse brain and cultured motoneurons." dans: Histochemistry and cell biology, Vol. 146, Issue 4, pp. 489-512, (2017) (PubMed).

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Anti-Rat IgG whole molecule antibody generated in goat detects specifically Rat IgG whole molecule. This secondary antibody anti-Rat is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.