Lapin anti-Rat IgG (Heavy & Light Chain) Anticorps

N° du produit ABIN102162

N° du produit (Fournisseur): 612-4126

Aperçu rapide pour Lapin anti-Rat IgG (Heavy & Light Chain) Anticorps (ABIN102162)

Antigène: IgG

Clonalité: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Détail du produit

Épitope: Heavy & Light Chain

Reactivité: Rat

Hôte: Lapin

N° du produit (Fournisseur): 612-4126

Fournisseur: Rockland

Fonction: Rat IgG (H&L) Antibody Pre-adsorbed

Réactivité croisée (Details): Minimal crossreactivity against Human Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Rat IgG and Rat Serum. No reaction was observed against Human Serum Proteins.

Attributs du produit: Anti-Rat IgM antibody generated in rabbit specifically detects rat IgM heavy chain.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Stérilité: Sterile filtered

Immunogène: Optional[Immunogen]: Anti-Rat IgG (H&L) was produced by repeated immunization with rat IgG whole molecule in rabbit

Information d'application

Indications d'application: Application Note: Anti-Rat IgG has been tested by ELISA and western blot and has been assayed against 1.0 μg of Rat IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: 1:1,000-1:5,000 Western Blot Dilution: 1:2,000-1:10,000 ELISA Dilution: 1:20,000 - 1:300,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 2.2 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Anti-Rat IgG (H&L) antibody generated in rabbit detects specifically rat IgG (H&L). This secondary antibody anti-Rat is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.