Lapin anti-Mouton IgG (Heavy & Light Chain) Anticorps

N° du produit ABIN102249

N° du produit (Fournisseur): 613-4102

Aperçu rapide pour Lapin anti-Mouton IgG (Heavy & Light Chain) Anticorps (ABIN102249)

Antigène: IgG

Clonalité: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Détail du produit

Épitope: Heavy & Light Chain

Reactivité: Mouton

Hôte: Lapin

N° du produit (Fournisseur): 613-4102

Fournisseur: Rockland

Fonction: Sheep IgG (H&L) Antibody

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Sheep IgG and Sheep Serum.

Attributs du produit: Anti-Sheep IgG F(ab')2 Rhodamine Antibody generated in rabbit recognizes the dimeric Fab portion of the sheep IgG molecule. Sheep IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind sheep IgG F(c) will not bind sheep IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Sheep IgG F(ab')2 Antibody is conjugated to Rhodamine.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose.

Stérilité: Sterile filtered

Immunogène: Optional[Immunogen]: Sheep IgG whole molecule

Information d'application

Indications d'application: Application Note: Anti-Sheep IgG antibody has been tested by ELISA and is suitable for use in ELISA, lateral flow, immunohistochemistry, and western blot. Specific conditions for reactivity should be optimized by the end user. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:50,000 - 1:200,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Anti-Sheep IgG (H&L) generated in rabbit detects sheep Immunoglobulin G. Both the Heavy and Light chains of the antibody molecule are present. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.