Lapin anti-Mouton IgG (Fc Region) Anticorps

N° du produit ABIN102286

N° du produit (Fournisseur): 613-4103

Aperçu rapide pour Lapin anti-Mouton IgG (Fc Region) Anticorps (ABIN102286)

Antigène: IgG

Clonalité: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)

Isotype: IgG

Détail du produit

Épitope: Fc Region

Reactivité: Mouton

Hôte: Lapin

N° du produit (Fournisseur): 613-4103

Fournisseur: Rockland

Fonction: Sheep IgG Fc Antibody

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Sheep IgG, Sheep IgG F(c) and Sheep Serum.  No reaction was observed against Sheep IgG F(ab).

Attributs du produit: Anti-Sheep IgG (H&L) generated in rabbit detects sheep Immunoglobulin G. Both the Heavy and Light chains of the antibody molecule are present. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Purification: Anti-Sheep IgG F(c) was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Anti-Sheep IgG was produced by repeated immunization with sheep IgG F(c) fragment in rabbit.

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: Anti-Sheep IgG F(c) antibody has been tested by ELISA and dot blot and is assayed against 1.0 μg of Sheep IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:10,000 - 1:200,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Receptors bind the Fc portion of sheep IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. Anti-Sheep IgG F(c) antibody is ideal for investigators in Cancer, Immunology, and Microbiology research.