Lapin anti-Mouton IgG (Fc Region) Anticorps (Alkaline Phosphatase (AP)) - Preadsorbed

N° du produit ABIN102298

N° du produit (Fournisseur): 613-4529

Aperçu rapide pour Lapin anti-Mouton IgG (Fc Region) Anticorps (Alkaline Phosphatase (AP)) - Preadsorbed (ABIN102298)

Antigène: IgG

Clonalité: Polyclonal

Conjugué: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Détail du produit

Épitope: Fc Region

Reactivité: Mouton

Hôte: Lapin

N° du produit (Fournisseur): 613-4529

Fournisseur: Rockland

Fonction: Sheep IgG Fc Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

Réactivité croisée (Details): Minimal crossreactivity against Human Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Sheep IgG, Sheep IgG F(c) and Sheep Serum.  No reaction was observed against Sheep IgG F(ab) or Human Serum Proteins..

Attributs du produit: Anti-Sheep IgG (H&L) Alkaline Phosphatase Antibody generated in rabbit detects reactivity to sheep IgG.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Stérilité: Sterile filtered

Immunogène: Optional[Immunogen]: Sheep IgG F(c) fragment

Information d'application

Indications d'application: Application Note: This product has been assayed against 1.0 μg of Sheep IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3,000 to 1:14,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Receptors bind the Fc portion of sheep IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.