Lapin anti-Souris IgG2a Anticorps (Biotin)

N° du produit ABIN102375

N° du produit (Fournisseur): 610-4641

Aperçu rapide pour Lapin anti-Souris IgG2a Anticorps (Biotin) (ABIN102375)

Antigène: IgG2a

Clonalité: Polyclonal

Conjugué: Biotin

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Isotype: IgG

Détail du produit

Reactivité: Souris

Hôte: Lapin

N° du produit (Fournisseur): 610-4641

Fournisseur: Rockland

Fonction: Mouse IgG2a (Gamma 2a chain) Antibody Biotin Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Mouse IgG and Mouse Serum.   Specificity was confirmed by ELISA.  Typically less than 1 % cross reactivity was observed against other Mouse heavy chain isotypes.

Attributs du produit: Anti-Mouse IgG1 Biotin Antibody generated in rabbit detects reactivity to Mouse IgG1 (Gamma 1 chain).

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogène: Optional[Immunogen]: Mouse IgG2a heavy chain

Information d'application

Indications d'application: Application Note: Anti-Mouse IgG2a Biotin Antibody has been tested by ELISA and is ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détail du antigène

Antigène: IgG2a

Classe de substances: Antibody

Sujet: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.