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Lapin anti-Cat IgM Anticorps

ELISA, IHC, WB Polyclonal IgG unconjugated
Rockland
N° du produit ABIN102560
N° du produit (Fournisseur): 202-4107

Aperçu rapide pour Lapin anti-Cat IgM Anticorps (ABIN102560)

Antigène

IgM

Clonalité

  • 517
  • 114
Polyclonal

Application

  • 354
  • 317
  • 304
  • 236
  • 167
  • 115
  • 91
  • 91
  • 64
  • 64
  • 36
  • 35
  • 26
  • 22
  • 21
  • 15
  • 9
  • 6
  • 5
  • 3
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype

IgG
  • Reactivité

    • 225
    • 168
    • 89
    • 40
    • 30
    • 29
    • 28
    • 22
    • 17
    • 14
    • 13
    • 13
    • 13
    • 12
    • 11
    • 4
    • 2
    • 1
    Cat

    Hôte

    • 353
    • 220
    • 99
    • 28
    • 14
    • 5
    • 4
    • 1
    • 1
    Lapin

    N° du produit (Fournisseur)

    202-4107

    Fournisseur

    Rockland

    Fonction

    Cat IgM mu chain Antibody

    Réactivité croisée (Details)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Cat IgM and Cat Serum.  No reaction was observed against Cat IgG.

    Purification

    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

    Immunogène

    Optional[Immunogen]: Cat IgM mu heavy chain
  • Indications d'application

    Application Note: Anti-Cat IgM antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 5.0 mL

    Concentration

    10.0 mg/mL

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    , Preservative:0.01 % (w/v) Sodium Azide

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

    Date de péremption

    12 months
  • Antigène

    IgM

    Classe de substances

    Antibody

    Sujet

    Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Cat IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.
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