Chèvre anti-Humain IgM Anticorps (Alkaline Phosphatase (AP))

N° du produit ABIN102625

N° du produit (Fournisseur): 609-1507

Aperçu rapide pour Chèvre anti-Humain IgM Anticorps (Alkaline Phosphatase (AP)) (ABIN102625)

Antigène: IgM

Clonalité: Polyclonal

Conjugué: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)

Isotype: IgG

Détail du produit

Reactivité: Humain

Hôte: Chèvre

N° du produit (Fournisseur): 609-1507

Fournisseur: Rockland

Fonction: Human IgM (mu chain) Antibody Alkaline Phosphatase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Human IgM and Human Serum.  No reaction was observed against other human heavy or light chain proteins.

Attributs du produit: Anti-Human IgA Alkaline Phosphatase Antibody generated in goat detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Purification: Anti-Human IgM mu heavy chain antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Human IgM whole molecule

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: Anti-Human IgM (mu heavy chain) Alkaline Phosphatase has been tested by dot blot and ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Date de péremption: 12 months

Détail du antigène

Antigène: IgM

Classe de substances: Antibody

Sujet: Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Anti-Human IgM mu chain antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.