Âne anti-Poulet IgG (Heavy & Light Chain) Anticorps (TRITC) - Preadsorbed

N° du produit ABIN1043928

N° du produit (Fournisseur): 603-700-c37

Aperçu rapide pour Âne anti-Poulet IgG (Heavy & Light Chain) Anticorps (TRITC) - Preadsorbed (ABIN1043928)

Antigène: IgG

Clonalité: Polyclonal

Conjugué: TRITC

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Isotype: IgG

Détail du produit

Épitope: Heavy & Light Chain

Reactivité: Poulet

Hôte: Âne

N° du produit (Fournisseur): 603-700-c37

Fournisseur: Rockland

Fonction: Chicken IgG (H&L) Antibody Rhodamine Conjugated Pre-Adsorbed

Réactivité croisée (Details): Minimal crossreactivity against Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Chicken IgG and Chicken Serum.   No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Chicken IgG and with light chains of most Chicken immunoglobulins.

Attributs du produit: Anti-Chicken IgG F(ab')2 Antibody generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind Chicken IgG F(c) will not bind Chicken IgG F(ab')2 Molecules.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by conjugation and extensive dialysis against the buffer stated above.

Immunogène: Optional[Immunogen]: Chicken IgG whole molecule

Information d'application

Indications d'application: Application Note: Anti-Chicken IgG Antibody Rhodamine conjugated has been tested by western blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.