Chèvre anti-Souris IgG2a Anticorps

N° du produit ABIN1043978

N° du produit (Fournisseur): 610-101-041

Aperçu rapide pour Chèvre anti-Souris IgG2a Anticorps (ABIN1043978)

Antigène: IgG2a

Clonalité: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)

Isotype: IgG

Détail du produit

Reactivité: Souris

Hôte: Chèvre

N° du produit (Fournisseur): 610-101-041

Fournisseur: Rockland

Fonction: Mouse IgG2a Antibody Pre-adsorbed

Réactivité croisée (Details): Minimal crossreactivity against to Bv, Hu & Rb Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum, Mouse IgG and Mouse IgG2a. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA for human and rabbit. For IgG1, IgG2b and IgG3 reactivity was less than 5 % against target (IgG2a, IgG). Cross-reactivity against Bovine is assessed only by IEP.

Attributs du produit: Anti-Mouse IgG1 Antibody generated in goat detects reactivity to Mouse IgG1 (Gamma 1 chain).

Purification: MOUSE IgG2a Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG2a coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Mouse IgG2a heavy chain

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: Anti-Mouse IgG2a secondary antibody has been tested by ELISA and dot blot and is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG2a antibody based assays. Mouse IgG2a secondary antibody is available in a variety of formats. Immunohistochemistry Dilution: 1:2,000 - 1:10,000 Western Blot Dilution: 1:4,000 - 1:20,000 ELISA Dilution: 1:40,000 - 1:200,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.1 mg/mL

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, Preservative:0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: IgG2a

Classe de substances: Antibody

Sujet: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.