Chèvre anti-Lapin IgG (Heavy & Light Chain) Anticorps (Alkaline Phosphatase (AP))

N° du produit ABIN1044051

N° du produit (Fournisseur): 711-105-002

Aperçu rapide pour Chèvre anti-Lapin IgG (Heavy & Light Chain) Anticorps (Alkaline Phosphatase (AP)) (ABIN1044051)

Antigène: IgG

Fragment: F(ab')2 fragment

Clonalité: Polyclonal

Conjugué: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Détail du produit

Épitope: Heavy & Light Chain

Reactivité: Lapin

Hôte: Chèvre

N° du produit (Fournisseur): 711-105-002

Fournisseur: Rockland

Fonction: F(ab')2 Rabbit IgG (H&L) Antibody Alkaline Phosphatase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Rabbit IgG and Rabbit Serum.  No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).

Attributs du produit: F(ab')2 Anti-Rabbit IgG (H&L) Antibody generated in goat detects immunoglobulin g from Rabbit, both heavy and light chains of the antibody molecule are present. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays. This F(ab')2 Anti-Rabbit IgG Antibody is conjugated to Peroxidase.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation.

Stérilité: Sterile filtered

Immunogène: Optional[Immunogen]: Rabbit IgG whole molecule

Information d'application

Indications d'application: Application Note: F(ab')2 Anti-Rabbit IgG Alkaline Phosphatase Conjugated Antibody has been tested by ELISA and is assayed against 1.0 μg of Rabbit IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: 1:200-1:1,000 Western Blot Dilution: 1:500-1:2,500 ELISA Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Optional[Buffer]: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stock: 4 °C

Stockage commentaire: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.