WB, FACS, FLISA, FM
Polyclonal
IgG
TRITC
Fab fragment
N° du produit ABIN1044085
N° du produit (Fournisseur): 807-4002
Aperçu rapide pour Lapin anti-Hamsters dorés syriens IgG (Heavy & Light Chain) Anticorps (TRITC) (ABIN1044085)
Antigène
IgG
Fragment
Fab fragment
Clonalité
Polyclonal
Conjugué
TRITC
Application
Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
Isotype
IgG
Épitope
Heavy & Light Chain
Reactivité
Hamsters dorés syriens
Hôte
Lapin
N° du produit (Fournisseur)
807-4002
Fournisseur
Rockland
Fonction
Fab Golden Syrian Hamster IgG (H&L) Antibody Rhodamine Conjugated
Réactivité croisée (Details)
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Rabbit IgG F(c).
Attributs du produit
Fab Anti-Golden Syrian Hamster IgG (H&L) Antibody generated in goat detects hamster IgG. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Fab Antibody is ideal for investigators who routinely perform flow cytometry, immunofluorescence, IHC, and other immunoassays. This Fab Anti-Golden Syrian Hamster IgG Antibody is conjugated to Texas Red™.
Purification
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Golden Syrian Hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
Immunogène
Optional[Immunogen]: Golden Syrian Hamster IgG whole molecule
ELISA, IHC, WB
Polyclonal
IgG
Alkaline Phosphatase (AP)
F(ab')2 fragment
Indications d'application
Application Note: Fab Anti-Golden Syrian Hamster IgG (H&L) Antibody has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:500-1:2,500 Other: FLOW CYTOMETRY 1:500 - 1:2,500
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL
Concentration
1.0 mg/mL
Buffer
Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stock
4 °C,-20 °C
Stockage commentaire
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Date de péremption
12 months
Antigène
IgG
Classe de substances
Antibody
Sujet
Fab Anti-Golden Syrian Hamster IgG (H&L) Antibody generated in rabbit detects hamster IgG. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Fab Antibody is ideal for investigators who routinely perform flow cytometry, immunofluorescence, IHC, and other immunoassays. This Fab Anti-Golden Syrian Hamster IgG Antibody is conjugated to Rhodamine.