Chèvre anti-Souris IgG2a Anticorps (Biotin)

N° du produit ABIN1118481

N° du produit (Fournisseur): 610-106-041

Aperçu rapide pour Chèvre anti-Souris IgG2a Anticorps (Biotin) (ABIN1118481)

Antigène: IgG2a

Clonalité: Polyclonal

Conjugué: Biotin

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Isotype: IgG

Détail du produit

Reactivité: Souris

Hôte: Chèvre

N° du produit (Fournisseur): 610-106-041

Fournisseur: Rockland

Fonction: Mouse IgG2a Antibody Biotin Conjugated Pre-adsorbed

Réactivité croisée (Details): Minimal crossreactivity against to Bv, Hu, and Rb Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Mouse Serum and Mouse IgG2a. Specificity was confirmed by ELISA at less than 1 % cross-reactivity against other mouse heavy or light chain isotypes. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1 % of target signal.

Attributs du produit: Anti-Mouse IgG1 Biotin Antibody generated in goat detects reactivity to Mouse IgG1 (Gamma 1 chain).

Purification: MOUSE IgG2a Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogène:

Immunogen: Mouse IgG2a heavy chain

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: Mouse IgG2a Biotin Antibody has been tested by ELISA and is available in a variety of formats. Anti-Mouse IgG2a biotin conjugated secondary antibody is suitable for ELISA, Immunohistochemistry, western blotting as well as other anti IgG2a antibody based assays. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store second antibody at 4° C prior to restoration. For extended storage aliquot secondary antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Référence

Jia, Xu, Smeets, Buhl, Moeller, Floege, Klinkhammer, Boor: "The Role of Platelet-Derived Growth Factor in Focal Segmental Glomerulosclerosis." dans: Journal of the American Society of Nephrology : JASN, Vol. 34, Issue 2, pp. 241-257, (2023) (PubMed).

Détail du antigène

Antigène: IgG2a

Classe de substances: Antibody

Sujet: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.