Chèvre anti-Souris IgG3 Anticorps (Alkaline Phosphatase (AP)) - Preadsorbed

N° du produit ABIN1118506

N° du produit (Fournisseur): 610-105-043

Aperçu rapide pour Chèvre anti-Souris IgG3 Anticorps (Alkaline Phosphatase (AP)) - Preadsorbed (ABIN1118506)

Antigène: IgG3

Clonalité: Polyclonal

Conjugué: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)

Isotype: IgG

Détail du produit

Reactivité: Souris

Hôte: Chèvre

N° du produit (Fournisseur): 610-105-043

Fournisseur: Rockland

Fonction: Mouse IgG3 Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

Réactivité croisée (Details): Minimal crossreactivity against to Bv, Hu, and Rb Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Alkaline Phosphatase, Mouse Serum and Mouse IgG3. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1 % of target signal.

Attributs du produit: Anti-Mouse IgG2b Alkaline Phosphatase Antibody generated in goat detects reactivity to Mouse IgG2b (Gamma 2b chain).

Purification: Anti-MOUSE IgG3 (Gamma 3 chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Mouse IgG3 heavy chain

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: Anti-Mouse IgG3 Alkaline Phosphatase Antibody has been tested by ELISA and dot blot and is ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods. Alkaline Phosphatase Conjugated Secondary Antibodies are ideal for western blot and are available in a variety of formats and conjugate types. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.1 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store vial at 4° C before opening. DO NOT FREEZE. Conjugated antibody is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Date de péremption: 12 months

Détail du antigène

Antigène: IgG3

Classe de substances: Antibody

Sujet: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG3 comprises almost 10 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.