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Lapin anti-Humain IgM (Fc5mu Region) Anticorps (Alkaline Phosphatase (AP)) - Preadsorbed

ELISA, IHC, WB, DB Polyclonal Alkaline Phosphatase (AP)

N° du produit ABIN117295

Fiche de données

Antigène Tous les produits IgM

IgM
Épitope
141

117

87

41

17

8

7

1

1
Fc5mu Region
Reactivité
192

134

66

39

32

26

22

21

16

15

15

14

13

13

12

4

2

1
Humain
Hôte
321

188

78

19

14

5

4

1

1
Lapin
Clonalité
496

87
Polyclonal
Conjugué
121

77

76

56

36

32

24

15

12

11

11

11

10

9

9

9

7

5

5

4

4

4

4

4

4

4

4

4

4

4

4

3

3

3

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1
Alkaline Phosphatase (AP)
Application
338

250

239

211

143

139

64

62

47

47

47

42

38

29

20

18

13

6

5

5

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)

Purification

Preadsorption: Solid phase absorption

Immunogène

Human IgM (Fc5µ) fragment
Mouse anti-Human IgM antibody (HRP)
ELISA, WB Monoclonal CH2 IgG1 HRP

Goat anti-Human IgM (Heavy & Light Chain) antibody - Preadsorbed
ELISA, IHC, WB Polyclonal IgG unconjugated

Goat anti-Human IgM antibody (Biotin)
ELISA, FACS Polyclonal IgG Biotin F(ab')2 fragment

Goat anti-Human IgM antibody (FITC)
ELISA, FACS Polyclonal IgG FITC F(ab')2 fragment

Goat anti-Human IgM (Heavy Chain) antibody (FITC)
ELISA, IHC, FACS Polyclonal IgG FITC

Goat anti-Human IgM (Heavy Chain) antibody (TRITC)
ELISA, IHC, FACS Polyclonal IgG TRITC

Goat anti-Human IgM (Heavy Chain) antibody (Texas Red (TR))
ELISA, IHC, FACS Polyclonal IgG Texas Red (TR)

Goat anti-Human IgM antibody (HRP) - Preadsorbed
ELISA, FLISA Polyclonal IgG HRP

Goat anti-Human IgM (Heavy Chain) antibody (Alkaline Phosphatase (AP))
ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)

Goat anti-Human IgM antibody (HRP)
ELISA, FACS, FLISA Polyclonal IgG HRP F(ab')2 fragment

Indications d'application

Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry aswell as other phosphatase-antibody based enzymatic assays requiring lot-to-lotconsistency. Recommended Dilutions: This product has been assayed against 1.0 μg of Human IgG in astandard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutesat room temperature. A working dilution of 1:500 to 1:2,000 of the reconstitutionconcentration is suggested for this product.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

1.0 mg/mL

Buffer

0.05 M Tris Chloride, 0.15 M Sodium Chloride, 0.005 M Magnesium Chloride, 0.0005 M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0 with 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative.

Agent conservateur

Sodium azide

Précaution d'utilisation

WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation

Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Stock

4 °C

Stockage commentaire

Store the antibody (undiluted) at 2-8 °C.
Antigène

IgM

Abstract

IgM Produits

Classe de substances

Antibody

Sujet

Synonyms: Human Immunoglobulin M