Chèvre anti-Humain Ig (Chain lambda), (Light Chain) Anticorps (Biotin)
Aperçu rapide pour Chèvre anti-Humain Ig (Chain lambda), (Light Chain) Anticorps (Biotin) (ABIN117720)
Antigène
Fragment
Clonalité
Conjugué
Application
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Épitope
- Chain lambda, Light Chain
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Reactivité
- Humain
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Hôte
- Chèvre
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Attributs du produit
- Molar Ratio: 10-20 BAC molecules per Goat IgG Fab molecule.
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Purification
- Immunoaffinity chromatography.
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Immunogène
- Human lambda (lambda) light chain
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Indications d'application
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Suitable for Immunoblotting, ELISA, Immunohistochemistry, immunomicroscopy as well asother antibody based assays using streptavidin or avidin conjugates requiring extremelylow background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titerand specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of Human IgG in astandard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6- sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 4,000 to 1: 20,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Reconstitution
- Restore with 1.0 mL of deionized water (or equivalent).
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Concentration
- 1.0 mg/mL (by UV absorbance at 280 nm)
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Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01 % (w/v) Sodium Azide as preservative.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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- Ig
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Autre désignation
- Immunoglobulin
Antigène
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